Engineering Transcriptional Regulator Effector Specificity Using Computational Design and In Vitro Rapid Prototyping: Developing a Vanillin Sensor

Engineering Transcriptional Regulator Effector Specificity Using Computational Design and In Vitro Rapid Prototyping: Developing a Vanillin Sensor.

The pursuit of circuits and metabolic pathways of increasing complexity and robustness in synthetic biology will require engineering new regulatory tools. Feedback control based on relevant molecules, including toxic intermediates and environmental signals, would enable genetic circuits to react appropriately to changing conditions. In this work, variants of qacR, a tetR family repressor, were ...

Engineering Transcriptional Regulator E↵ector Specificity using Computational Design and In Vitro Rapid Prototyping: Developing a Vanillin Sensor

The pursuit of circuits and metabolic pathways of increasing complexity and robustness in synthetic biology will require engineering new regulatory tools. Feedback control based on relevant molecules, including toxic intermediates and environmental signals, would enable genetic circuits to react appropriately to changing conditions. In this work, variants of qacR, a tetR family repressor, were ...

Fabrication of Tissue Engineering Using Rapid Prototyping

Rapid prototyping (RP) techniques are a group of advanced manufacturing processes that can produce custom made objects directly from computer data such as Computer Aided Design (CAD), Computed Tomography (CT) and Magnetic Resonance Imaging (MRI) data. Using RP fabrication techniques, constructs with controllable and complex internal architecture with appropriate mechanical properties can be ach...

Rapid Prototyping in Biomedical Engineering

Rapid Prototyping Your Sensor Network

Several considerable impediments stand in the way of sensor network prototype applications that wish to realize sustained deployments. These are: scale, longevity, data of interest, and infrastructure integration. We present a tool, Que, which assist those sensor network deployments transitioning from prototypes to early production environments by addressing these issues. Que is able to simulat...